Signal intensity must correlate with the quantity – possibly mass or concentration – with the detected sample with the presented time position, making it possible for the quantification and identification of the separated analytes in the time-dependent manner. The level of retardation predominantly relies on the nature in the analyte and also

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The reaction surface area analyze type, a CCD with 11 operates, was utilized. The proposed CCD experimental design was used along with the analysis of mobile period composition and pH of buffer was performed from the 3 responses, retention time, peak place, and symmetry aspect; the final results are summarized in Table 2 and Figure four, respective